Performance analysis of orthogonal pairs designed for an expanded eukaryotic genetic code.
The suppression of amber stop codons with non-canonical amino acids (ncAAs) is used for the site-specific introduction of many unusual functions into proteins.Specific orthogonal aminoacyl-tRNA synthetase (o-aaRS)/amber suppressor tRNA(CUA) pairs (o-pairs) for the incorporation of ncAAs Security in S.cerevisiae were previously selected from an E.co